Skip to main content
. Author manuscript; available in PMC: 2010 May 19.
Published in final edited form as: Biochemistry. 2009 May 19;48(19):4159–4169. doi: 10.1021/bi900224c

Figure 8. Effect of GSTP1a-1a expression on rosiglitazone-induced PPARγ-dependent transcription.

Figure 8

Parental GST-poor MCF7/WT (- GSTP1-1) and transgenic cells expressing GSTP1a-1a, MCF/π-11 (+ GSTP1-1) were transiently co-transfected with the PPARγ-responsive PPRE-3x-TK-Luc reporter, the control pGL4.75 reporter, and the pcDNA3-PPARγ expression plasmids. Transfected cells were treated with 0.5 μM rosiglitazone or vehicle control as described in Experimental Procedures. Fold induction was calculated from corrected firefly luciferase activies as outlined in Figure 4. Bars represent the mean values ± 1 sd from 9 independent transfections.