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. Author manuscript; available in PMC: 2009 Jul 13.
Published in final edited form as: Mol Cell. 2007 Aug 3;27(3):486–497. doi: 10.1016/j.molcel.2007.06.015

Figure 5. TCR substrates as Sts-1 targets.

Figure 5

(A) Dephosphorylation of Zap-70 by Sts-1 PGM. Zap-70 was immuno-precipitated from activated Jurkat T cells and utilized as a substrate. Reaction products were evaluated by anti-phosphotyrosine western analysis, following which the blot was re-probed with Zap-70 antibodies. Sts-1* is an inactive Sts-1PGM mutant in which the two His and two Arg within the active site are mutated to Ala.

(B) Dephosphorylation of tyrosine phosphorylated proteins downstream of the TCR. Proteins from TCR-stimulated Jurkat cells were isolated by immunoprecipitation, eluted from the pTyr antibodies, and evaluated as Sts-1PGM substrates. Sts-1* is described in (A).

(C) Effect of wild-type vs. catalytically inactive Sts-1 on TCR-induced tyrosine phosphorylation. Jurkat T cells transfected to express wild-type or inactive Sts-1 (Sts-1 H380,565A) were stimulated with anti-CD3 antibodies, and tyrosine phosphorylation was evaluated by anti-pTyr immunoblot analysis.