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. Author manuscript; available in PMC: 2009 Aug 15.
Published in final edited form as: J Immunol. 2008 Aug 15;181(4):2330–2337. doi: 10.4049/jimmunol.181.4.2330

FIGURE 3.

FIGURE 3

Rescue of defective JNK activation and CXCL-8 expression in MIF/D-DT-deficient cells by adenoviral reconstitution of MIF and D-DT. A, A549 lung adenocarcinoma cells were oligo transfected and 48 hours later GFP adenovirus or MIF + D-DT adenovirus was added as indicated. 24 hours after adenovirus addition, cells were lysed and immunoblotted for MIF, D-DT, p-JNK, total JNK and β-actin. B, A549 cells were transfected with scrambled, MIF, D-DT or MIF + D-DT siRNA oligos for 48 hours and adenoviral MIF and/or D-DT was added to cells. 24 hours later, media was replaced and collected the following day for CXCL-8 ELISA measurements. ELISA results are expressed ± S.D. of the average of data from two samples and are representative of 2 independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001 by one way ANOVA analysis is shown for control and individual group comparisons while statistical significance between relevant groups was found by Tukey’s post hoc test.