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. Author manuscript; available in PMC: 2009 Aug 15.
Published in final edited form as: J Immunol. 2008 Aug 15;181(4):2330–2337. doi: 10.4049/jimmunol.181.4.2330

FIGURE 5.

FIGURE 5

Adenoviral reconstitution of MIF and/or D-DT rescues defective tube formation from supernatants derived from MIF + D-DT-deficient cells. A549 lung adenocarcinoma cells were oligo transfected and 48 hours later GFP, MIF, D-DT or MIF + D-DT adenovirus was added as indicated. After overnight incubation, media was replaced and 48 hours later conditioned media was collected for HUVEC tube formation. Representative GF-depleted Matrigel coated wells were photographed (A) and enumerated, (B). Results from panel B are tubes/transwell and represent the ± S.D. of the average of parallel co-culture assays and are representative of 3 independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001 by one way ANOVA analysis is shown for control and individual group comparisons while statistical significance between relevant groups was found by Tukey’s post hoc test.