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. Author manuscript; available in PMC: 2009 Aug 15.
Published in final edited form as: J Immunol. 2008 Aug 15;181(4):2330–2337. doi: 10.4049/jimmunol.181.4.2330

FIGURE 6.

FIGURE 6

MIF and D-DT are individually and additively sufficient to induce CXCL-8 from lung adenocarcinoma cells and CD74 is necessary for maximal JNK activation in mouse fibroblasts. A, A549 lung adenocarcinoma cells were oligo transfected with either Control siRNA (NS) or CD74 siRNA and 48 hours later GFP, MIF, D-DT or MIF + D-DT adenovirus was added for 16 hours. After media was replaced, cells were incubated for an additional 24 hours and supernatants were collected and analyzed by CXCL-8 ELISA. Results are the ± S.D. of the average of CXCL-8 ELISA readings from supernatants from two parallel infections and are representative of 2 independent experiments. B, CD74+/+ and CD74−/− murine embryonic fibroblasts were infected with GFP, MIF, D-DT or MIF + D-DT for 48 hours. Cell lysates were examined for pJNK, p-c-jun and total JNK. Results are representative of 2 independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001 by one way ANOVA analysis is shown for control and individual group comparisons while statistical significance between relevant groups was found by Tukey’s post hoc test.