Table 1. Transcript names, short names, accession numbers, primer sequences, amplicon sizes, and fold change expression values obtained by microarray and qRT–PCR for selected transcripts.

Transcript name	Short name	Accession Number	Forward primer	Reverse primer	Amplicon size	FC by microarray	FC by qRT–PCR
40S ribosomal protein S20	RPS20	BG936672	GCAGACCTTATCCGTGGAGCTA	TGGTGATGCGCAGAGTCTTG	85
HSP90-beta	HSP90B	AF135117	CTCTGGGATGAGCTCCTCACA	CCTTTGACCTCTTTGAGAACAAGAA	98
Elongation factor 1AA	EF1AA	AF321836	CCCCTCCAGGACGTTTACAAA	CACACGGCCCACAGGTACA	57
Elongation factor 1AB	EF1AB	BG933853	TGCCCCTCCAGGATGTCTAC	CACGGCCCACAGGTACTG	59
Metallothionein B	MT-B	CK990996	TGAATAAAGAAGCGCGATCAAA	CTGGTGCATGCGCAGTTG	111	1.8	1.5*
Fatty acid binding protein	FABP2	CB511332	TTACGGAAGGTGCTGGATTC	GGGCATCAATGTGATGAAGA	108	−2.2	−2.5*
Gamma crystallin M2	CryG M2	CB510188	GGAGAAGATGTCACGGTGGT	CCCCCTACAGAGGAGCCTAC	125	−1.8	−1.8*
Ependymin precursor	EPN	CA042089	TCCAGTTTAGCGTCCTGACA	GACAAGACCGGCTGGATACT	104	−1.7	−2.3*
Fructose-bisphosphate aldolase B	Aldo b	CA043730	CCTGTGTGGTCATCTCTCCAT	TGACAAGGGTGTCCTCTTCC	116	−1.7	−1.1
Sodium/potassium-transporting ATPase subunit alpha-1 precursor	ATPA1C	CA054630	AGGGAGACGTACTACTAGAAAGCAT	CAGAACTTAAAATTCCGAGCAGCAA	85	1.4	1.5*
Calpain small subunit 1	CAPNS1	CB505442	AGGCACCCAATGAAGTTGTC	CTCAGGGCTCATCTCCTCAC	144	1.4	1.5*
Glyceraldehyde-3-phosphate dehydrogenase	GAPDH	CA051897	GGTATCCCTTCATGGGTCCT	CGTGTCAGTGGTGGACCTAA	104	1.2	1.8*
Ubiquitin-conjugating enzyme E2 D4	UbE2D4	CB508919	CATTGCATACTTTTGAGTCCAATC	ACCCAGATGACCCCCTAGTC	101	−1.3	−1.4*
Cold-inducible RNA-binding protein	Cirbp	CA062835	TGAGCTTCGACACCACAGAG	ACGAGACCTTCCCGTTTCTT	104	1.3	1.1
Peroxiredoxin-6	PRDX6	CA062947	TCAGGGATGTTTGGAGGAAC	GGGGCGTAACTTTGATGAGA	126	−1.2	−1.1
SPARC precursor	SPARC	CA052160	CCAAGGCGATGTACTTGTCA	GGTTCCTGTCCCACACAGAG	117	1.3	1.3*
Lens fiber membrane intrinsic protein	LIM2	CA039129	CCAGGAAGTTCACCGTCACT	TAATCGCAGGCATTCTCTCC	147	−1.3	−1.3*
Heat shock cognate 70 kDa protein	HSC71	CA767816	ACCTCGTTGCACTTCTCCAG	GCAGCGTGACAAGGTCTCTT	138	1.2	1.2*
Ferritin. heavy subunit	Ferritin H	CA052852	GTGTGGGTCGTTGTGTTCAG	AGTGGGGTAGTGGTGTGGAG	110	1.2	−1
Phospholipid hydroperoxide glutathione peroxidase. mitochondrial precursor	GPX4	CB505439	GGCTGTTCCTTCATCCACTT	GCCAGGTACAGAGGTGGAAA	126	1.2	1.1

The first four rows in the table contain the selected reference genes that are not differentially expressed in lenses from the different dietary groups. Expression of the 16 transcripts in the subsequent rows was significantly different between the dietary groups when analyzed by microarray and SAM, but only 11 of the transcripts were significantly different when analyzed by qRT–PCR analysis and Mann–Whitney test (FCs are marked with an asterisk in the table).