Figure 2. Kinetics of ROS versus IL-8 production in P. acnes-stimulated keratinocytes.

HaCaT cells were incubated with P. acnes (MOI of 50) for a period of time ranging from 0.25 to 24 h. (A) Superoxide anion, (B) nitric oxide, (C) hydrogen peroxide levels were determined by spectrofluorometry as described in Materials and Methods. (D) IL-8 production was determined by ELISA as described in Materials and Methods. Data are means±SD of two separate experiments.