Figure 7. Relationship between ROS and IL-8 productions in P. acnes-stimulated keratinocytes.

HaCaT cells were incubated for 18 h with P. acnes alone (MOI of 50) (black bar) or with P. acnes in the presence of ROS modulators (gray bars). The concentrations used were 40 µM rotenone, 40 µM antimycin, 40 µM allopurinol, 40 µM DPI, 2 mM DDC, 400 µM ATZ, 0.8 mM BSO, 100 µM manganese[III]tetrakis(5,10,15,20)-benzoic acid porphyrin (MnTBAP), 400 µM copper[II]diiosopropylsalicylate (CuDIPS), 3.2 mM N-acetylcysteine (NAC), 1.6 mM GSH, 20 U catalase. IL-8 concentration was measured in culture supernatants by ELISA as described in Materials and Methods. Data are means±SD of two separate experiments.