Figure 8. P. acnes induces the production of superoxide anions via CD36.

HaCaT cells were pretreated 2 h with human anti-TLR-2 or human anti-CD36 monoclonal antibodies (black bars), goat anti-IgG antibody (white bar) and incubated 3 h with P. acnes (A_{600 nm} = 1.0). Control experiments were run in parallel with P. acnes alone (gray bar). (A) IL-8 concentrations were measured in culture supernatants by ELISA, as described in Materials and Methods and are showed minus the value obtained with the HaCaT cells treated with the mAb alone. (B) O₂ ^•− production was measured spectrofluometrically over a period of 3 h as described in Materials and Methods. Data are presented as means±SD of three separate experiments.