Skip to main content
. 2009 Jul 22;4(7):e6334. doi: 10.1371/journal.pone.0006334

Figure 4. IGFBP-3 modulates the effects of HN on insulin sensitivity.

Figure 4

A) IGFBP-3 binding of HN and HN analogues measured by densitometry of dot blots probed with radiolabeled IGFBP-3 (Ins = Insulin). B) Mouse embryonic fibroblasts generated from wild-type or IGFBP-3 knockout mice were incubated in serum-free media for 24 h followed by incubation with 100 nM HNG or HNGF6A for 24 h. Apoptosis was assessed by ELISA for fragmentation of histone-associated DNA. (n = 4). Effects of ICV IGFBP-3, HN or F6AHN during a hyperinsulinemic clamp (n = 6 each)on C) GIR, D) HGP and E) degree of suppression of HGP. *Significantly different from other experimental groups, P<0.05.