Table 1. MOS4-associated proteins identified by mass-spectrometry.
| AGI | Protein | H. sapiens | S. cerevisae | S. pombe | NTC Reference |
| NTC Core Proteins | |||||
| AT1G09770 | AtCDC5/MAC1; R2-R3 Myb transcription factor | CDC5L | Cef1p | Cdc5p | [18], [20]–[25],[43],[51] |
| AT1G04510 | MAC3A; PUB; WD-40 repeat family | hPrp19/SNEV | Prp19p | Cwf8p | [18], [20]–[25],[43],[51] |
| AT2G33340 | MAC3B; PUB; WD-40 repeat family | hPrp19/SNEV | Prp19p | Cwf8p | [18], [20]–[25],[43],[51] |
| AT3G18165 | MOS4; protein-protein interactions | SPF27/BCAS2 | - - | Cwf7p | [18],[20],[24],[25],[43] |
| AT4G15900 | PRL1/MAC2; WD-40 repeat family | PLRG1/PRL1 | Prp46p/Cwc1p | Prp5p/Cwf1p | [18], [20], [22]–[25],[43],[51] |
| Other NTC associated proteins | |||||
| AT1G07360 | zinc finger (CCCH-type) family protein; RNA binding | RBM22 | Ecm2p | Ecm2p | [20], [23]–[25],[51] |
| AT1G77180 | chromatin protein family | SKIP/SNW | Prp45p | Prp45p/Cwf13p | [20], [22]–[25] |
| AT2G38770 | EMB2765; helicase | KIAA0560 | - - | Cwf11p | [24],[25] |
| AT3G18790 | similar to two coiled coil domains of eukaryotic ori | hIsy1 | Isy1p/Ntc30p | Cwf12p | [20]–[24] |
| AT5G28740 | transcription-coupled DNA repair-related; RNA processing | hSyf1/XAB2 | Syf1p | Cwf3p | [20]–[24],[51] |
| AT5G41770 | cell cycle control crooked neck protein-like; RNA processing | Clf1p/CRN/Syf3 | Clf1p/Syf3 | Cwf4p | [22],[24],[25],[51] |
| Predicted splicing-related proteins | |||||
| AT1G06220 | CLO/GFA1/MEE5; translation elongation; nucleic acid binding | U5-116kD | Snu114p | Cwf9p | [20], [22]–[25],[51] |
| AT1G10580 | WD-40 repeat family; nucleotide binding | Cdc40/Dhx38 | Prp17p/Cdc40p | Prp17p | [20], [22]–[25] |
| AT1G15200 | protein-protein interaction regulator family | pinin | - - | - - | [24] |
| AT1G20960 | EMB1507; DEAD-box helicase | U5-200kD | Brr2p | Brr2p | [20], [22]–[25] |
| AT1G32490 | EMB2733/ESP3; DEAD-box like helicase | hPrp2/DHX16 | Prp2p | Cdc28p | [22],[24] |
| AT1G80070 | SUS2/EMB177; embryogenesis | U5-220kD | Prp8p | Cwf6p | [20], [22]–[25],[51] |
| AT2G43770 | WD-40 repeat family; nucleotide binding | U5-40kD | - - | Cwf17p | [20],[22],[24] |
| AT5G64270 | putative splicing factor similar to RCN1; PP2A regulator | U2-SAP155 | Hsh155 | Sap155 | [18],[22],[25] |
| Unrelated proteins/Possible contaminants | |||||
| AT3G15730 | PLDα1; hormone response | - - | - - | - - | - - |
| AT3G20820 | LRR family; defense response | - - | - - | - - | - - |
| AT3G60190 | ADL1E/DRP1E/EDR3; GTPase; defense response | DNM1 | - - | - - | - - |
| AT4G19410 | putative pectin acetylesterase; carboxylesterase activity | - - | - - | - - | - - |
| AT5G42080 | ADL1A/DRP1A/RSW9; GTPase | DNM1 | - - | - - | - - |
Nuclear extracts from complementing mos4-1 plants expressing PMOS4-MOS4-HA were immunoprecipitated using anti-HA beads, separated by SDS-PAGE and silver-stained (Figure 1C). The MOS4-HA IP lane was cut into eight pieces, digested, and the proteins contained were analyzed by mass-spectrometry. Sequenced peptides were used as queries in BLAST searches against the 2007 version of the Arabidopsis genome. Proteins with strong or partial homology to Arabidopsis proteins from other eukaryotes are listed in the columns to the right. Proteins are divided into sections according to their sequence homology to known NTC- or NTC-associated proteins, based on a previous computational organization [34]. Dashes indicate that no proteins with significant homology were identified by BLAST. Abbreviations: ADL: Arabidopsis dynamin-like protein; BCAS: Breast carcinoma sequence; BRR: Bad response to refrigeration; CDC: Cell division cycle; CEF1: S. cerevisiae homolog of cdc5(+); CLF: Crooked neck-like factor; CLO: Clotho; CRN: Crooked neck pre-mRNA splicing factor-like; CWC: Complexed with Cef1p; CWF: Complexed with Cdc5p; DHX: DEAH-box; DNM: Dynamin; DRP: Dynamin-related protein; EDR: Enhanced disease resistance; EMB: Embryo lethal; ESP: Enhanced silencing phenotype; GFA: Gametophytic factor; HSH: Human Sap homolog; ISY: Interactor of Syf; LRR: Leucine-rich repeat; MAC: MOS4-associated complex; MEE: Maternal effect embryo arrest; MOS: Modifier of snc1; NTC: Nineteen complex; PLDα: Phospholipase D alpha; PP2A: Protein phosphatase type 2A; PRL/PLRG: Pleiotropic regulating locus; PRP: Precursor mRNA processing; RBM: RNA binding motif; RCN: Roots curl in NPA; RSW: Radial swelling; SAP: Splicing associated protein; SKIP: Ski-interacting protein; SNEV: Senescence evasion factor; SNU: Small nuclear ribonucleoprotein associated; SNW: “SNW” domain; SPF: Spliceosome-associated protein; SUS: Abnormal suspensor; SYF: Synthetic lethality with Cdc40; XAB: XPA-binding protein.