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. Author manuscript; available in PMC: 2010 Apr 28.
Published in final edited form as: Vaccine. 2009 Feb 24;27(19):2616–2624. doi: 10.1016/j.vaccine.2009.02.051

Figure 7.

Figure 7

The murine phrenic nerve-hemidiaphragm was used to assay the ability of anti-light chain antibodies to neutralize the actions of botulinum toxin type A (final concentration, 3 × 10-12 M). When botulinum toxin alone was added to control tissues (○;n=6), evoked responses decayed and a 90% reduction in twitch amplitude occurred within ca. 120 min. Essentially the same outcome was obtained when toxin was pre-incubated with pre-immune serum before being added to tissue baths (▲;n=3). When toxin was pre-incubated with anti-light chain antiserum (10 μl; ■;n=3), the actions of the toxin were blocked during the entire observation period. The standard error of the mean for each data point in the figure was equal to or less than 4% of the mean for the data point