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. 2000 Sep 26;97(20):10751–10756. doi: 10.1073/pnas.97.20.10751

Figure 3.

Figure 3

Northern blot analysis of ER mRNAs in tissues of mature female (n = 3 for α and γ; n = 2 for β) and mature male (n = 2, testes only) Atlantic croaker. L, liver; O, ovary; B, brain; M, muscle; T, testes. Amounts of mRNA loaded per tissue type: liver, ovary, testes = 3 μg per lane; brain = 2 μg per lane; muscle = 1.3 μg per lane. Lanes shown are one of three loads of mRNA from the same fish. (A) Atlantic croaker ERα (acERα). (B) Atlantic croaker ERβ (acERβ). (C) Atlantic croaker ERγ (acERγ). The size (kb) of each transcript is indicated. RNA size markers (mk) are shown (Millennium Markers, Ambion).