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. Author manuscript; available in PMC: 2009 Jul 13.
Published in final edited form as: J Mol Cell Cardiol. 2007 May 18;43(3):243–253. doi: 10.1016/j.yjmcc.2007.05.008

Figure 3. RNA expression of zbmper in whole mount zebrafish embryos.

Figure 3

(A–C and E–G) Embryos were analyzed by in situ hybridization using a zbmper specific probe [purple]. (A) Dorsal view of a 24 hpf zebrafish embryo. Zbmper is expressed in the lateral dorsal aorta (lda) associated with the aortic arches as well as within a cranial transverse connection (c), the eye primordia (e), and the dorsal midline (dm) of the head area. (B and C) Higher magnifications of the yolk sac extension. (B) Oblique dorsolateral view, anterior to the left. zbmper is expressed in the ICM (*) located at the distal end of the yolk sac extension. (C) Dorsal view, anterior to the left. Zbmper is expressed in the paraxial ICM more concentrated at the distal end of the yolk sac extension.(D) Dorsolateral view of a 24 hpf flk1:GFP transgenic fish embryo shows flk:GFP expression in the symmetric lateral dorsal aorta (lda) and anterior cardinal vein (acv) as well as in the transverse connection (c). Anterior to the left. (E) Dorsolateral view of a 48 hpf zebrafish embryo. Zbmper is strongly expressed in the lateral dorsal aorta and the aortic arches. Weaker expression is detectable in the pineal gland (pg) and in the eye lenses (el). (F) Transverse section at the level of the aortic arches at 48 hpf. As observed in whole mount preparations zbmper is strongly expressed in the lateral dorsal aorta associated with the aortic arches. Zbmper is also expressed in the lateral mesoderm (lm) that hosts early vascular precursor cells in vertebrate organisms. (G) Transverse section at the level of the dotted line in (E). Zbmper is expressed around the ventral wall of the dorsal aorta but not in the axial vein. (nc) notochord. (da) dorsal aorta. (av) axial vein. (y) yolk sac.