Figure 4. Loss of zbmper causes a dorsalized phenotype.

(A) Western blot of zebrafish embryo lysates at 36hpf. Zbmper is effectively knocked down in embryos displaying the characteristic phenotype. (B–D) Lateral views of zebrafish embryos at different developmental stages. (B) Tail of a control zebrafish embryo at 48 hpf. (C) Tail of a representative zbmper morphant embryo at 36 hpf. The shape of the tail indicates a dorsalized phenotype. Somitic segments distal of the dorsal tail kick are smaller than in controls and are dysmorphic. Blood cells are pooled in the dorsal aorta proximal to the tail deformation (arrowhead). (D) Tail of a zbmper morphant embryo at 72 hpf. The tail has adopted a pigtail shape indicating a severely dorsalized phenotype.