Table 1.
LC-MS/MS identification of proteins in DRMs isolated from myc-tagged CA-OsRac1-transformed rice suspension cells
| Protein | Accession No. | Mol. wt (kDa) | MASCOT score | No. of hit peptides | Sequence coverage (%) | TM predictiona | |
|---|---|---|---|---|---|---|---|
| Innate immunity | Phosphatidylinositol-4-phosphate 5-kinase family protein, low similarity to phosphatidylinositol 3,5-kinase | gi|115467450 | 175 | 61 | 2 | 1 | 0 |
| Disease resistance protein RPM1 (CC-NBS-LRR class), putative domain signature CC-NBS-LRR exists | gi|125535287 | 140 | 61 | 2 | 1 | 0 | |
| NB-ARC domain, putative | gi|115484771 | 110 | 58 | 2 | 2 | 0 | |
| Respiratory burst oxidase protein D (RbohD)/NADPH oxidase identical to respiratory burst oxidase protein D | gi|108864453 | 105 | 186 | 12 | 23 | 5 | |
| Leucine-rich repeat transmembrane protein kinase, putative protein kinase Xa21 | gi|125532138 | 103 | 60 | 2 | 1 | 2 | |
| Respiratory burst oxidase protein D (RbohD)/NADPH oxidase identical to respiratory burst oxidase protein D | gi|115488934 | 100 | 108 | 9 | 14 | 5 | |
| Heat shock protein 90 | gi|6863054 | 93 | 80 | 4 | 6 | 0 | |
| Serine/threonine protein kinases active site signature | gi|32988727 | 76 | 62 | 2 | 3 | 1 | |
| Atypical harpin-like kinase MARK | gi|13324792 | 72 | 548 | 14 | 32 | 1 | |
| Heat shock cognate 70 kDa protein | gi|115452223 | 71 | 305 | 10 | 14 | 0 | |
| Nodulation harpin kinase-like protein | gi|115478014 | 59 | 129 | 4 | 9 | 1 | |
| Putative elicitor inducible beta-1,3-glucanase NtEIG-E76 | gi|115439837 | 54 | 100 | 9 | 18 | 1 | |
| Putative disease resistance protein | gi|115434084 | 48 | 75 | 2 | 5 | 0 | |
| Putative protein kinase serine/threonine protein kinase, putative similar to Pto kinase interactor 1 (Pti1) | gi|115456539 | 41 | 54 | 2 | 9 | 0 | |
| Chitin elicitor-binding protein | gi|108860575 | 40 | 218 | 8 | 27 | 2 | |
| Putative Pto kinase interactor 1 | gi|115461953 | 39 | 75 | 6 | 19 | 0 | |
| Receptor for activated C kinase 1A (RACK1A) | gi|115439261 | 36 | 115 | 3 | 13 | 0 | |
| Putative hypersensitive-induced response protein | gi|115465785 | 32 | 477 | 25 | 48 | 0 | |
| Band 7 family protein strong similarity to hypersensitive-induced response protein | gi|115482396 | 32 | 212 | 11 | 32 | 0 | |
| Hypersensitive-induced response protein | gi|14150732 | 31 | 106 | 4 | 9 | 0 | |
| Hypersensitive-induced response protein | gi|115476296 | 31 | 337 | 21 | 40 | 0 | |
| Small GTP-binding protein OsRac2 | gi|115464861 | 24 | 59 | 2 | 16 | 0 | |
| Harpin-induced family protein (YLS9)/HIN1 family protein | gi|115461434 | 22 | 179 | 3 | 25 | 2 | |
| Harpin-induced gene 1 homolog | gi|2801538 | 22 | 92 | 6 | 30 | 2 | |
| Harpin-induced protein 1-containing protein | gi|115487442 | 21 | 154 | 6 | 27 | 2 | |
| Harpin-induced family protein/NDR1/HIN1-like protein 3 similar to harpin-induced protein hin1 | gi|32993161 | 21 | 93 | 3 | 23 | 2 | |
| Harpin-induced protein-related/HIN1-related/harpin-responsive protein-related weak similarity to hin1 | gi|115472507 | 19 | 76 | 2 | 11 | 0 | |
| Receptor | Putative receptor-like protein kinase | gi|115467902 | 94 | 61 | 5 | 6 | 2 |
| Leucine-rich repeat family protein, expressed | gi|108863916 | 84 | 81 | 9 | 12 | 2 | |
| Leucine-rich repeat transmembrane protein kinase, putative | gi|116012935 | 74 | 211 | 4 | 11 | 1 | |
| Somatic embryogenesis protein kinase 1 | gi|52854318 | 70 | 59 | 5 | 8 | 2 | |
| Brassinosteroid-insensitive 1-associated harpin kinase 1 precursor (somatic embryogenesis harpin-like kinase 3) | gi|115458750 | 70 | 59 | 4 | 5 | 1 | |
| Leucine-rich repeat transmembrane protein kinase, putative similar to CLV1 harpin kinase | gi|115459088 | 66 | 59 | 3 | 7 | 1 | |
| Protein kinase family protein, putative, expressed | gi|115450539 | 59 | 131 | 5 | 13 | 0 | |
| Putative kinase-like protein TMKL1 precursor | gi|115438420 | 54 | 81 | 2 | 4 | 2 | |
| Leucine-rich repeat family protein similar to leucine-rich repeat protein SHOC-2 (Ras-binding protein Sur-8) | gi|32975440 | 51 | 79 | 2 | 5 | 0 | |
| Peptidoglycan-binding LysM domain-containing protein contains Pfam profile PF01476 | gi|115480519 | 39 | 164 | 4 | 14 | 2 | |
| SNARE | Syntaxin 121, putative, expressed | gi|115455787 | 36 | 56 | 2 | 5 | 1 |
| Putative syntaxin-related protein (SYP132) | gi|115470721 | 34 | 189 | 6 | 20 | 1 | |
| Synaptobrevin family protein similar to vesicle-associated membrane protein 7 | gi|125557559 | 32 | 156 | 9 | 25 | 2 | |
| Novel plant SNARE 11 | gi|115453189 | 31 | 65 | 12 | 30 | 1 | |
| Syntaxin 71 (SYP71) identified as syntaxin of plants 71 | gi|115465329 | 30 | 199 | 14 | 47 | 1 | |
| Vesicle-associated membrane protein 724 | gi|115456011 | 25 | 53 | 2 | 11 | 1 | |
| Small G-protein and others | WD-40 repeat family protein contains three WD-40 repeats (PF00400); some similarity to s-tomosyn isoform | gi|156765316 | 168 | 343 | 21 | 20 | 0 |
| WD-40 repeat family protein contains three Pfam PF00400: WD domain, G-beta repeats | gi|37988349 | 148 | 582 | 29 | 25 | 0 | |
| Probable GTP-binding protein | gi|115437816 | 90 | 89 | 2 | 5 | 0 | |
| Transducin family protein/WD-40 repeat family protein | gi|108706574 | 82 | 606 | 33 | 34 | 0 | |
| Transducin family protein/WD-40 repeat family protein contains six WD-40 repeats; similar to cell cycle control protein cwf8 | gi|115482422 | 58 | 64 | 8 | 21 | 0 | |
| GTP-binding protein GTP1 | gi|115439941 | 24 | 59 | 3 | 8 | 0 | |
| ADP-ribosylation factor, putative, expressed | gi|108711707 | 22 | 87 | 3 | 18 | 0 |
aThe numbers of putative transmembrane domains were predicted by SOSUI.