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. 2009 Apr 28;37(12):3897–3911. doi: 10.1093/nar/gkp261

Figure 1.

Figure 1.

Upregulation of FGFR3 expression by BMP-2 treatment in C3H10T1/2 cells. (A, B) C3H10T1/2 cells were incubated with the indicated dosage of BMP-2 with 5% FBS for 1 day (A), or for the indicated periods of time in the presence of 200 ng/ml BMP-2 with 5% FBS (B) and total RNA was extracted for measurement of FGFR3 mRNA expression level by semi-quantitative RT–PCR. *P < 0.05; **P < 0.01 versus the corresponding untreated cells. All the results are the means of three independent experiments ± standard deviation. (C) C3H10T1/2 cells were pretreated with control or SB203580 (10 μM) for 30 min and cultured for another 30 min in the presence or absence of BMP-2 (200 ng/ml). The whole lysates were examined by immunoblotting with antibodies against phosphorylated p38 and total p38. (D) C3H10T1/2 cells were pretreated with SB203580 (10 μM) or CHX (10 μM) for 30 min, followed by addition of BMP-2 (200 ng/ml) for 1 day in the continued presence of CHX or SB203580. FGFR3 mRNA expression level was examined by semi-quantitative RT–PCR. The PCR products were electrophoresed on agarose gel and stained with ethidium bromide.