Figure 4.

Metabolite profiles in unfed and glucose-fed SP cells. (A) Relationship of glycolysis to acetyl-CoA production for use by nuclear KATs. (B) ¹H-NMR spectra of a glucose reference sample (top panel) and samples of metabolites isolated from SP cells (bottom panel; only glucose peaks are shown). (C) Targeted quantitative profiling of cellular metabolite levels by ¹H-NMR, before and after glucose refeeding. Identical volumes were analyzed from samples prepared from the same number of cells and resuspended in the same final volume. The results are the average of two independent experiments. (D) Cellular level of acetate in glucose-fed cells relative to the level in unfed cells (average of three independent experiments ± SD). Acetate was measured by an enzymatic assay.