Table 3.
Activity assays of reaction systems in which mutations were engineered into the exogenous IGS triplets
| IGS mutations | Relative yield in V•X•Y + h•Z self-assembly reaction (%) | Relative activity in W•X•Y•Z ribozyme (%) | Relative activity in V•X•Y•Z ribozyme (%) |
|---|---|---|---|
| (wild type) | 100 | 100 | 100 |
| GUG2 | 0 | 59 | 2.5 |
| GUG4 | 0 | 125 | 60 |
| GUG5 | 0 | 102 | 71 |
| GUG2/GUG4 | 0 | 1.5 | 5.0 |
| GUG2/GUG5 | 0 | Trace | 2.4 |
| GUG4/GUG5 | 0 | 4.6 | 45 |
| GUG2/GUG4/GUG5 | 0 | Trace | 8.8 |
Table values are relative bipartite covalent self-assembly yields after 3 h (second column) or splicing assays using an exogenous substrate after 1 h (third and fourth columns), normalized to that of the unmutated construct, set at 100%. Trace = less than 1% product detected. 0% = no V•X•Y•Z product detected.