Table 2.
Sensitivity of ΔpnpA and recombination-deficient strains upon acute exposure to H2O2 and MMS
| Relevant phenotype (epistatic group) | Lethal concentration to kill 90% of the cells (LC90)a |
|||
|---|---|---|---|---|
| H2O2 (in mM) |
MMS (in mM) |
|||
| pnp+ | ΔpnpA | pnp+ | ΔpnpA | |
| wt (NA) | 13.5 ± 0.1 | 10.0 ± 0.3 | 17.8 ± 0.1 | 27.1 ± 0.09 |
| ΔrecA (NA) | 0.20 ± 0.03 | 0.11 ± 0.02 | 0.41 ± 0.04 | 0.30 ± 0.02 |
| ΔrecO (α) | 0.30 ± 0.02 | 0.19 ± 0.01 | 0.73 ± 0.08 | 0.54 ± 0.10 |
| ΔrecR (α) | 0.31 ± 0.02 | 0.20 ± 0.03 | 0.72 ± 0.09 | 0.54 ± 0.08 |
| addA5 (β) | 0.35 ± 0.03 | 0.19 ± 0.02 | 7.1 ± 0.07 | 7.8 ± 0.08 |
| ΔrecN (δ) | 0.7 ± 0.04 | 5.2 ± 0.1 | 8.8 ± 0.14 | 9.8 ± 0.15 |
| ΔrecU (ϵ) | 0.31 ± 0.02 | 0.30 ± 0.04 | 0.67 ± 0.10 | 1.25 ± 0.10 |
| ΔrecQ (ζ) | 9.8 ± 0.4 | 7.0 ± 0.2 | 16.6 ± 0.08 | 31.1 ± 0.19 |
| ΔrecJ (ζ) | 9.6 ± 0.3 | 7.1 ± 0.2 | 16.7 ± 1.9 | 30.8 ± 1.2 |
| ΔrecG (η) | 0.27 ± 0.04 | 0.48 ± 0.02 | 0.63 ± 0.09 | 1.21 ± 0.14 |
| Δku (NA) | 10.2 ± 0.1 | 15.9 ± 0.3 | 16.1 ± 0.1 | 30.9 ± 0.1 |
The single and double mutant strains were exposed to increasing concentrations of the genotoxic agent for 15 min, and dilutions were plated on LB agar plates and incubated overnight at 37°C. Standard errors are indicated. Figures S4 and S5A show the primary data from where the data in Table 2 was derived. NA: not applied.
aThe LC90 results were the average of at least three independent experiments.