Table 3.
Growth of different strains on plates containing different concentrations of MMS, H2O2, MMC or 4NQO
| Strain | No drug | H2O2 (mM) | MMS (mM) | MMC (pM) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 0.2 | 0.5 | 1 | 0.07 | 1.2 | 2.7 | 5 | 90 | 180 | |||||
| wt | ++++ | ++++ | ++++ | ++++ | ++++ | ++++ | ++ | ++++ | +++ | − | |||
| ΔpnpA | ++++ | ++++ | ++++ | (+++) | ++++ | ++++ | +++ | ++++ | ++++ | ++++ | |||
| ΔrecO | +++ | (++) | − | − | (+++) | − | − | (+++) | − | − | |||
| ΔrecO ΔpnpA | ++ | + | − | − | (+) | − | − | (++) | − | − | |||
| addA5 | ++++ | ++++ | (++) | − | ++++ | (++) | − | ++++ | − | − | |||
| addA5 ΔpnpA | +++ | +++ | (+) | − | ++++ | (+++) | − | ++++ | (+) | − | |||
| ΔrecQ | ++++ | ++++ | ++++ | +++ | ++++ | ++++ | (++) | ++++ | +++ | − | |||
| ΔrecQ ΔpnpA | ++++ | ++++ | +++ | (++) | ++++ | ++++ | +++ | ++++ | ++++ | ++++ | |||
| ΔrecN | ++++ | ++++ | (+++) | (+) | ++++ | +++ | (+) | ++++ | (+) | − | |||
| ΔrecN ΔpnpA | +++ | ++++ | (+++) | + | ++++ | +++ | + | ++++ | (+++) | + | |||
| ΔrecU | (+++) | (+++) | − | − | (+++) | − | − | +++ | − | − | |||
| ΔrecU ΔpnpA | (++) | (+) | − | − | − | − | − | (+) | − | − | |||
| ΔrecG | +++ | + | − | − | + | − | − | (+++) | − | − | |||
| ΔrecG ΔpnpA | (+++) | (++) | − | − | (++) | − | − | (+++) | − | ||||
| Strain | No drug | H2O2 (mM) | MMS (mM) | MMC (pM) | 4NQO (nM) | ||||||||
| 0.2 | 0.5 | 1 | 0.07 | 1.2 | 2.7 | 5 | 90 | 180 | 7.5 | 50 | 200 | ||
| wt | ++++ | ++++ | ++++ | ++++ | ++++ | ++++ | ++ | ++++ | +++ | − | ++++ | (++++) | ++ |
| ΔpnpA | ++++ | ++++ | ++++ | (+++) | ++++ | ++++ | +++ | ++++ | ++++ | ++++ | ++++ | ++++ | +++ |
| Δku | ++++ | ++++ | ++++ | ++++ | ++++ | ++++ | ++ | ++++ | +++ | − | ++++ | (++++) | ++ |
| Δku ΔpnpA | ++++ | ++++ | ++++ | (+++) | ++++ | ++++ | +++ | ++++ | +++ | ++++ | ++++ | ++++ | +++ |
| ΔrecA | ++ | (++) | − | − | ++ | − | − | (+++) | − | − | (++) | − | − |
| ΔrecA ΔpnpA | (++) | (+) | − | − | (+) | − | − | (++) | − | − | (+) | − | − |
| ΔrecA Δku | (++) | (++) | − | − | ++ | − | − | (++) | − | − | (++) | − | − |
Growth of different strains on plates containing the indicated concentrations of MMS, H2O2 or MMC. Symbols: normal growth, ++++; compromised growth, +++; compromised growth and small colony, (+++); poor growth with normal size colonies, ++; compromised growth and small colony, (++); poor growth and tiny colony, +; extremely poor growth and tiny colony, (+); no growth, −. Results are the average of at least three independent experiments. Drug concentrations that highlight the differences between strains are shown. Figures S3 and S5B show the primary data from which the data in this table were derived.