Table 1.
Parameters used to fit the qPCR data
| U (V) | q*/e | P0 (s–1) | A (counts) | |
|---|---|---|---|---|
| TGAATTC | 2.16 ± 0.08 | 0.98 ± 0.06 | 6.74 ± 0.83 | 3.9 ± 5.2 |
| 2.08 ± 0.03 | 0.99 ± 0.22 | 3.46 ± 0.62 | 6.1 ± 2.1 | |
| 2.09 ± 0.06 | 1.01 ± 0.12 | 1.91 ± 0.25 | 24.8 ± 15.3 | |
| AGAATTC | 1.62 ± 0.07 | 0.98 ± 0.24 | 4.61 ± 0.43 | 5.3 ± 8.4 |
| 1.72 ± 0.05 | 0.91 ± 0.17 | 3.06 ± 0.57 | 11.2 ± 15.5 | |
| 1.84 ± 0.05 | 0.99 ± 0.17 | 2.33 ± 0.51 | 1.6 ± 5.0 | |
| TTAATTC | 1.17 ± 0.09 | 0.85 ± 0.10 | 11.5 ± 1.8 | 7.6 ± 7.2 |
| 1.18 ± 0.06 | 0.61 ± 0.15 | 4.11 ± 0.82 | 6.6 ± 5.5 | |
| 1.16 ± 0.03 | 0.97 ± 0.22 | 3.15 ± 0.68 | 5.3 ± 4.8 | |
| TGACTTC | 1.11 ± 0.08 | 1.00 ± 0.17 | 6.63 ± 1.38 | 12.1 ± 10.8 |
| 1.08 ± 0.04 | 1.02 ± 0.37 | 1.11 ± 0.51 | 7.1 ± 3.4 | |
| 1.02 ± 0.08 | 0.52 ± 0.08 | 2.32 ± 0.03 | 2.0 ± 6.2 | |
| TGCATTC | 0.87 ± 0.06 | 0.99 ± 0.11 | 7.01 ± 0.60 | 12.6 ± 5.1 |
| 0.93 ± 0.05 | 0.57 ± 0.08 | 5.57 ± 0.75 | 1.9 ± 1.0 | |
| 0.93 ± 0.06 | 0.59 ± 0.06 | 2.72 ± 0.05 | 12.8 ± 6.1 |
U denotes the threshold voltage, q* the effective charge, P0 is the capture rate and A is the qPCR baseline. The error reflects the distribution obtained from different fits to the same data. 3.4 × 4.7 ± 0.2 nm pore; 2.6 × 3.0 ± 0.2 nm pore; 2.5 ± 0.2 nm pore.