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. 2009 May 8;37(12):4089–4099. doi: 10.1093/nar/gkp326

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© 2009 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — RecA filament formation and dissociation on ssDNA as measured by magnetic tweezers. (a) Schematic drawing of the magnetic tweezers setup. (b) A 8.6 kb ssDNA molecule is extended by 48% compared to B-form DNA at a stretching force of 6 pN in the presence of 1 µM RecA, 1 mM ATP and 5 mM Mg²⁺. (c) Linear disassembly profile of a 8.6 kb RecA–ssDNA filament recorded at 0.5 pN. The black line is a guide to the eye.