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. 2009 May 5;37(12):3996–4009. doi: 10.1093/nar/gkp332

Figure 3.

Figure 3.

Effect of FHL2 on E47-mediated transcription inhibited by Id proteins. (A–E) MCF-7, 293T and SH–SY5Y cells were transiently cotransfected with pRL-SV40 and the indicated vectors. The relative luciferase activity levels were normalized in all cases by 5xE-box-Luc and mock effector transfection and arbitrarily assigned a value of 1. All experiments were performed in triplicate and were repeated at least three times, and the results are expressed as mean ± SEM. (F) MCF-7 cells were cotransfected with 5xE-box-Luc, pRL-SV40 and control-siRNA or FHL2-specific siRNA. The relative luciferase activity levels were normalized by 5xE-box-Luc and control-siRNA transfection and arbitrarily assigned a value of 1. All experiments were performed in triplicate and were repeated at least three times, and the results are expressed as mean ± SEM. (#P < 0.01).