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. 2009 May 5;37(12):3996–4009. doi: 10.1093/nar/gkp332

Figure 7.

Figure 7.

Downstream targets of E47–Id2 transcriptome are regulated by FHL2 in SK–N–SH neuroblastoma cells. (A) SK–N–SH cells were transiently transfected with the FHL2 expression vector or empty vector and cultured for the indicated times. Expression of the indicated transcript abundance was analyzed by qPCR values. HPRT was used as an internal control. All experiments were repeated at least three times, and the results are expressed as mean ± SEM. FHL2, Id2, E47, p57Kip2 and β-actin protein levels in transfected SK–N–SH cells analyzed by western blot is shown at the bottom. (B) SK–N–SH cells were transiently cotransfected with the indicated combinations of plasmids expressing E47, Id2 and FHL2. Cell lysates were analyzed for the expression of p57Kip2, IGF2, E47, Id2, FHL2 and β-actin. (C) SK–N–SH cells were transiently transfected with the indicated expression vector or different combinations. After 48 h transfection, total RNA was extracted and the abundance of p57Kip2 was analyzed by qPCR. HPRT was used as an internal control. All experiments were repeated at least three times, and the results are expressed as mean ± SEM (*P < 0.05; N, P > 0.05).