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Half formation time of the fully intercalated [TC3]4 tetramer versus the oligonucleotide concentration at 0°C pH 6.8 (black circles), 5.5 (open squares), 4.56 (black squares) and 3.6 (open circles). The lines drawn through the data points show that the half reaction time increases as power of −2 of the oligonucleotide concentration and therefore establish that the reaction order is 3.
