Figure 2.

The N terminus of LZIP contains a potent activation domain. (A) 293T cells were cotransfected with expression plasmids (25 ng) encoding Gal4_DBD, Gal4-LZIP_N154, Gal4-Sp1, and Gal4-VP16 together with the p5xGal-E1B-luc reporter gene (500 ng). Extracts were prepared after 36 h and assayed for luciferase activity. Values represent means and standard deviations of three independent transfections. (B) The boundaries of the activation domain were delineated with a series of C-terminal truncations. BHK21 cells were cotransfected with expression plasmids (250 ng) encoding Gal4_DBD, Gal4-LZIP_N154, Gal4-LZIP_N92, Gal4-LZIP_N77, and Gal4-LZIP_N46 together with the p5xGal-E1B-luc reporter gene (500 ng).