Figure 2.

Immunofluorescence of histamine H3 receptors in mouse Scarpa's ganglion. A: light microscope photomicrograph of a Scarpa's ganglion slice. H3 receptors appeared expressed in neurons characterized by large nuclei. White arrows point at three such neurons. B: confocal light photomicrograph of an enlarged image from a different ganglion slice. C: light microscope photomicrograph from a different ganglion slice, following H3R antibody preabsorption (see Methods). No labeling for H3R is detectable. Green: H3R antibody; Blue: DAPI. Slice thickness: 10 μm. Calibration bar: 20 μm.