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. 2009 Jun 29;10:70. doi: 10.1186/1471-2202-10-70

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Copyright © 2009 Tritto et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Immunofluorescence of calretinin and histamine H3 receptor in mouse Scarpa's ganglion. A: confocal image of a Scarpa's ganglion slice immunolabeled for H3 receptors. B: Immunolabeling for calretinin, same field as in A. C: nuclei counterstained with DAPI, same field as in A and B. D: overlapped images; yellow indicates the colocalization of calretinin and H3 receptors (three examples shown by arrows). Calibration bar: 10 μm. Cryostat slices thickness: 5 μm. Green: H3R Alexa Fluor 488; Red: Calretinin Alexa Fluor 546; Blue: DAPI. a, b, c and d are larger magnifications from a different slice, same antibodies and description as above. Calibration bar: 20 μm.