Figure 4.

Immunofluorescence of calbindin and histamine H3 receptor in mouse Scarpa's ganglion. All confocal images refer to the same field. A: slice immunolabeled for H3 receptors. B: Immunolabeling for calbindin. Note that, although staining is less homogeneous than in A, there is a substantial overlap of the stained soma. C: nuclei counterstained with DAPI. D: overlapped images; yellow indicates the co-localization of calbindin and H3R. Calibration bar: 20 μm. Cryostat slices thickness: 5 μm. Green: H3R Alexa Fluor 488; Red: Calbindin Alexa Fluor 546; Blue: DAPI.