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. Author manuscript; available in PMC: 2010 Jan 13.
Published in final edited form as: Biochemistry. 2009 Jan 13;48(1):41–49. doi: 10.1021/bi8015923

Figure 2. Binding of wild-type and mutant ERdj3 to γHC.

Figure 2

(A) Schematic representation of the domain composition of various ERdj3 mutants. (B) COS cells were transfected with the indicated ERdj3 constructs in the HA-DSL vector, metabolically labeled and immunoprecipitated with anti-ERdj3 or Protein A Sepharose alone. (C) COS cells were cotransfected with γHC and either wild-type ERdj3 or the indicated ERdj3 mutants. Cells were metabolically labeled, treated with DSP before lysing and immunoprecipitated with Protein A Sepharose to isolate γHC or with a monoclonal anti-HA antibody to isolate ERdj3 proteins. Samples were analyzed by reducing SDS-PAGE, and mutants that show diminished binding are indicated with arrows.