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. 2009 Jun 17;10:47. doi: 10.1186/1471-2121-10-47

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Copyright © 2009 Singh and Gramolini; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Validation of the interaction of TWIST with TCF-4 using immunoprecipitation assays. (A) Expression of wild type TWIST was shown by Western blotting using the anti-V5 antibody, (B) HEK293 cells were co-transfected with plasmids expressing V5-HIS-TWIST and CBP-TCF-4. Two different CBP antibodies were used (clone ID; K-24 and W-15). W-15 was used for the immunoblot. IgG beads with protein and antibody, and beads without antibody were used as positive and negative controls, respectively. Nuclear extracts were immunoprecipitated with anti-CBP tag antibodies (K-24 and W-15) bound resin, and the precipitated proteins were detected using anti-V5 antibody.