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. 2009 Jun 17;10:47. doi: 10.1186/1471-2121-10-47

Table 3.

Oligonucleotides used for mutagenesis reactions to alter NLSs sequences in TWIST.

Amino acid substituted in NLSs Primer Sequence
K38R 5'CGCGGGGGACGCAGGCGGCGCAGCAGC3'
5'GCTGCTGCGCCGCCTGCGTCCCCCGCG3'
K73R 5'CCCAGGGCAGGCGCGGCAAGAAGT3'
5'ACTTCTTGCCGCGCCTGCCCTGGG3'
K76R 5'CAAGCGCGGCAGGAAGTCTGCGG3'
5'CCGCAGACTTCCTGCCGCGCTTG3'
K77R 5'GCGGCAAGAGGTCTGCGGGCT3'
5'AGCCCGCAGACCTCTTGCCGC3'

K, lysine; R, arginine