Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Jul;20(7):1623–1632. doi: 10.1681/ASN.2008111148

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2009 by the American Society of Nephrology

PMC Copyright notice

Figure 2. — CHGA_1–40-induced secretion of EDN1 from human endothelial cells. (A) Effect of time. Results are presented as mean ± SEM over three measurements. Data are shown as net EDN1 release (CHGA_1–40 minus mock stimulation). HUVECs were incubated with 1 μM CHGA_1–40 for the indicated times. (B) Effect of dose. Results are presented as mean ± SEM over three measurements. Data are shown as net EDN1 release (CHGA_1–40 minus mock stimulation). HUVECs were incubated with the indicated concentrations of CHGA_1–40 for 15 min. (C) Dependence on extracellular calcium. HUVECs were incubated for 15 min with the indicated concentrations of CHGA_1–40, with or without calcium in the medium. Release of EDN1 from unstimulated controls was set as 100%. Data are shown as fold release over control. Results are presented as mean ± SEM over three measurements. (D) Probing a serotonin pathway. HUVECs were incubated for 15 min with the indicated concentrations of agonists (CHGA_1–40 or serotonin), with or without serotonin antagonist (cyproheptadine). Release of EDN1 from unstimulated controls was set as 100%. Data are shown as fold release over control. Results are presented as mean ± SEM over three measurements.