Table 2.

Stimulation of sGC with CO and YC-1

Conditions	Specific activity, nmol/min per mg	Stimulation, -fold
Basal	4.4  ± 0.5	1
CO	15.7  ± 0.7	4
CO + YC-1 (10 μM)	347  ± 32	78 (22.1)
CO + YC-1 (100 μM)	979  ± 34	221 (62.3)

All experiments were performed on samples saturated with CO (or argon for basal measurements) as described in Materials and Methods. Mg²⁺ was used as cofactor. The values for the specific activity are means (±SD) of three independent experiments performed for α^Nβ. α^Cβ enzyme showed a similar activity and stimulation (data not shown). Values in parentheses represent -fold activation by YC-1 over the CO stimulation.