Table 1.
Time (b) | [57Co]-Cbl uptake1 (fmol/mg protein) | [57Co]-Cofactor biosynthesis2 (fmol/mg protein) | ||
---|---|---|---|---|
Conditioned medium | Intracellular | AdoCbl | MeCbl | |
6 | 10.5 | 0.36 | 0.006 ± 0.002 | 0.003 ± 0.001 |
12 | 8.8 | 0.44 | 0.035 ± 0.005 | 0.007 ± 0.002 |
24 | 8.4 | 0.54 | 0.044 ± 0.014 | 0.008 ± 0.001 |
48 | 8.3 | 0.59 | 0.090 ± 0.021 | 0.012 ± 0.003 |
72 | 8.5 | 0.60 | 0.113 ± 0.016 | 0.008 ± 0.002 |
Uptake results represent three pooled samples per time point.
Cofactor biosynthesis is expressed as mean ± standard deviation (n=3)