Figure 2.

Inhibition of HIF-1α expression by 1. (a) After treating 4T1 cells under hypoxia (0.5% O2, 24 h) with and without 1, HIF-1α expression was evaluated using Western blots. By comparison with loading control (histone H1), nuclear expression of HIF-1α was significantly inhibited by 1 at concentrations higher than 10 nM. (b) In 4T1 and MDA-MB-231 cells, HIF-1α expression induced by CoCl2 (240μM, 24 h) was also inhibited by 1.