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Inhibition of VEGF secretion by 1. Inhibition of VEGF was determined in 4T1 cells after hypoxia treatment (0.5% O2, 24 h). After treatment with and without 1, cell culture supernates were collected, and VEGF secretion was measured using ELISA. Compound 1 significantly inhibited VEGF at concentrations higher than 10 nM (p<0.001).
