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. 2009 Jul 7;2009:910783. doi: 10.1155/2009/910783

Figure 1.

Figure 1

Effect of high glucose on PPAR γ expression. Rat glomerular mesangial cells were cultured in 5.6 mM (NG) or 25 mM D-glucose (HG), or 5.6 mM D-glucose + 24.4 mM L-glucose (LG) for up to 48 hours. (a) PPARγ was detected by immunobloting in total cell lysates, using β-actin as the loading controls. The graphs represent PPARγ protein levels relative to NG. (b) PPARγ mRNA levels were determined by real-time RT-PCR. (n = 4–6, *P < .05 versus NG; **P < .01 versus NG). (c) Preincubated with 10 μM rosiglitazone (RSG) or/and 10 μM GW9662, the protein expression of PPARγ was not affected by RSG or GW6992 in HG (n = 5, *P < .05 versus NG). Mesangial cells were transiently transfected with a luciferase reporter gene containing three PPAR response elements and then cultured in the above conditions. (d) Luciferase reporter activity was reduced in HG (n = 5, *P < .05 versus NG, **P < .01 versus NG). (e) In NG, PPARγ promoter activity increased in dose response to RSG (n = 4, *P < .01 versus NG). (f) GW6992 blocked the effect of RSG (n = 4–6, *P < .01 versus NG alone, **P < .05 versus NG or HG alone, ***P < .05 versus NG or HG with RSG).