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. 2009 Jul 7;2009:910783. doi: 10.1155/2009/910783

Figure 2.

Figure 2

Effect of rosiglitazone on reactive oxygen species generation. Mesangial cells were exposed to 5.6 mM (NG) or 25 mM (HG) D-glucose for up to 48 hours, and pretreated with 10 μM rosiglitazone (RSG) or GW9662 for 1 hour. Reactive oxygen species (ROS) were detected in DCF-loaded cells by confocal fluorescence imaging in 3 or 4 separate experiments for each condition. (a) ROS generation was inhibited by RSG (n = 147–218 cells, *P < .001 versus NG, **P < .001 versus HG 3 hours, HG 24 hours, or HG 48 hours). (b) ROS generation in NG was stimulated with GW9662 (n = 130–189 cells, *P < .001 versus NG, **P < .001 versus HG 48 hours without RSG or GW9662). Magnification bar = 25 μM.