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NADPH oxidase subunit p22phox expression. Mesangial cells were exposed to 5.6 mM (NG) or 25 mM (HG) D-glucose for 24 hours or 48 hours, or pretreated with 10 μM rosiglitazone (RSG), or 10 μM ciglitazone or 10 μM troglitazone) and p22phox was detected by immunoblotting. Pretreatment with three different PPARγ agonists blocked HG-induced p22phox expression (n = 5, *P < .05 versus NG, **P < .05 versus HG 24 hours or HG 48 hours).
