Figure 5.

Rosiglitazone prevented high glucose-induced collagen IV expression. Mesangial cells were exposed to 5.6 mM (NG) or 25 mM (HG) for up to 48 hours pretreated with 10 μM rosiglitazone (RSG) for 1 hour. (a) The expression of collagen IV (α1) mRNA was detected by real-time RT-PCR (n = 4, *P < .05 versus NG). (b) Representative confocal microscopic images show collagen IV staining without (C) or with RSG. Immunofluorescence intensity per cell was analyzed (n = 76–136 cells, **P < .01 versus NG). Magnification bar = 25 μM.