Figure 7.

Compound C reversed rosiglitazone prevented high glucose-induced reactive oxygen species generation. Mesangial cells were exposed to 5.6 mM (NG) or 25 mM (HG) for 3 hours or 48 hours, and then pretreated with 10 μM rosiglitazone (RSG) or 50 μM compound C (CPC), or combination of both, for 1 hour. Reactive oxygen species (ROS) generation was measured in DCF-loaded cells using confocal fluorescence imaging. RSG prevented high glucose stimulated ROS production that was abolished by coincubation with CPC (n = 145–204 cells, *P < .05 versus NG in the absence of RSG and/or CPC, **P < .01 versus HG in the absence of RSG and/or CPC). Magnification bar = 25 μM.