Figure 1.
Experimental design. Experiment 1, animals were subjected to the chronic unpredictable stress (CUS) procedure and tested in sucrose preference test (SPT), novelty suppressed feeding test (NSFT), forced swim test (FST) and active avoidance test (AAT). Experiment 4, animals were infused with saline, L-AAA or ibotenate and then subjected to the same 4 behavioral tests. Experiments 2 and 3 animals were infused with 50 and 100μg/μl of L-AAA into the PFC (Experiment 2) or with 100μg/μl of L-AAA or 5 μg/μl ibotenate (7 days after cannula implantation), and were then subjected to the SPT and FST immediately followed by a second SPT.