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. Author manuscript; available in PMC: 2009 Jul 14.
Published in final edited form as: Biochem Pharmacol. 2007 Jan 7;73(9):1499–1510. doi: 10.1016/j.bcp.2007.01.010

Fig. 4.

Fig. 4

Effects of NAC and DMTU on tubular cell apoptosis during cisplatin treatment. RPTC cells were incubated with 20 µM cisplatin for 16 h in the absence or presence of 10 mM NAC, 10 mM DMTU, or 10 mM Tiron. (A) Representative images of cell morphology. At the end of incubation, cells were stained with Hoechst 33342. Cellular and nuclear morphology was recorded by phase contrast and fluorescence microscopy, respectively. (B) Percentage of apoptosis. Apoptotic cells were examined by morphological methods and counted to assess the percentage of apoptosis. (C) Representative flow cytometric analysis of Annexin V-FITC and PI staining. (D) Caspase activity. Cell lysate was collected for enzymatic assay of caspase activity as described in Section 2. Data are expressed as mean ± S.D. (n = 4). *Statistically significantly different from the control group; #significantly different from the cisplatin-only group. The results show that NAC and DMTU suppressed cisplatin-induced caspase activation and apoptosis.