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. Author manuscript; available in PMC: 2009 Jul 14.
Published in final edited form as: Biochem Pharmacol. 2007 Jan 7;73(9):1499–1510. doi: 10.1016/j.bcp.2007.01.010

Fig. 5.

Fig. 5

Effects of DMTU on PUMA-α induction, Bax translocation, and cytochrome c translocation during cisplatin treatment. RPTC cells were incubated with 20 µM cisplatin for 16 h in the absence or presence of 10 mM DMTU. (A) PUMA-α induction. Whole cell lysate was collected for immunoblot analysis of PUMA-α. The blots were reprobed for β-actin to monitor protein loading and transferring. (B) Bax translocation. The cells were fractionated into the cytosolic and mitochondrial fractions for immunoblot analysis of Bax. (C) Cytochrome c release. The cells were fractionated into the cytosolic and mitochondrial fractions for immunoblot analysis of cytochrome c. Shown in this figure are representative blots of three separate experiments. The results show that DMTU attenuates PUMA-α induction and Bax translocation and cytochrome c release during cisplatin treatment of renal tubular cells.