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. Author manuscript; available in PMC: 2009 Jul 14.
Published in final edited form as: Radiat Res. 2009 Apr;171(4):454–463. doi: 10.1667/RR1329.1

FIG. 1.

FIG. 1

Schematic diagram of pCMS-end plasmid construct and NHEJ assay in mammalian cells. The plasmid pCMS-end was designed to monitor nonhomologous end joining (NHEJ) by FACS analysis. The multicloning site (MCS) is positioned between CMV enhancer/promoter region and coding sequence for EYFP; the EGFP sequence is under control of SV40 promoter (left). When the plasmid is digested with restriction enzyme(s) that only cut in MCS, linear DNA expresses EGFP but not EYFP (middle). Both colors can be detected by FACS analysis if repair of the DSB has taken place (right).