FIG. 4.

MMEJ events in yeast containing mismatches. The top line is the junction sequence recovered from the rejoined plasmids; the location of the sequence is indicated. The second line shows the sequence up-stream of the 5′ HindIII-linearized end. The third line shows the sequence at the site of MMEJ either close to the KpnI-linearized end or distant from the end. Overlapping sequences between lines 2 and 3 are shown as dashed lines, and the microhomologies used are shown as boldface letters. The mismatched nucleotides within the microhomologies are identified with a star on top of the nucleotides.