TABLE 1. End-Joining Events in Unirradiated and Irradiated Yeast Cells.
| Junctiona | Length of microhomology (bp)b | Overlapped basesc | Deletiond from |
||
|---|---|---|---|---|---|
| 5′ PSS end | 3′ PSS end | ||||
| Unirradiated clones | |||||
| C13 | 237-1226 | 5 | AGCTT | 0 | 953 |
| C2, C26 | 237-279 | 4 | AGCT | 0 | 6 |
| C18 | 237-1629 | 4 | AGCT | 0 | 1356 |
| C1, C27, C44 | 234-673 | 2 | TT | 3 | 400 |
| C8, C11, C17 | 234-676 | 2 | AG | 0 | 403 |
| C35 | 141-822 | 2 | GC | 96 | 549 |
| C4, C7, C14, C16, C22, C23, C36, C37 | 236-277 | 1 | G | 1 | 4 |
| C42 | 238-282 | 1 | A | 1 | 9 |
| C6, C32 | 134-277 | 1 | G | 103 | 4 |
| C20 | 142-274 | 1 | A | 95 | 1 |
| C25 | 237-279 | 0 | — | 0 | 6 |
| C37 | 236-277 | 0 | — | 1 | 4 |
| C9, C12, C29, C30, C38 | 237-276 | 0 | — | 0 ( 1) | 3 |
| C10 | 237-276 | 0 | — | 0 | 3 |
| C21, C41 | 237-281 | 0 | — | 0 | 8 |
| C40 | 237-282 | 0 | — | 0 | 9 |
| C3 | 142-277 | 0 | — | 95 | 4 |
| C24, C33 | 142-278 | 0 | — | 98 | 5 |
| Irradiated clones | |||||
| R24, R50 | 232-276 | 6 | AGCT*GG | 0 | 3 |
| R5, R11, R16, R17, R23, R25, R28, R30, R33, R34, R39, R41 | 237-673 | 4 | AG*TT | 0 | 400 |
| R14 | 236-747 | 4 | GCTT | 1 | 474 |
| R19 | 237-375 | 4 | AGCT | 0 | 102 |
| R9 | 142-280 | 3 | AGC | 95 | 7 |
| R26 | 143-676 | 3 | AAG | 94 | 403 |
| R29 | 219-441 | 2 | TC | 18 | 165 |
| R31 | 236-277 | 1 | G | 1 | 4 |
| R48 | 142-675 | 1 | A | 95 | 402 |
| R7 | 141-1023 | 0 | — | 96 | 750 |
| R8 | 142-276 | 0 | — | 95 | 3 |
| R32 | 141-276 | 0 | — | 96 | 3 |
| R15 | 229-273 | 0 | — | 8 | 2 |
| R6 | 237-277 | 0 | — | 0 | 4 |
| R35 | 236-276 | 0 | — | 1 | 3 |
| R36 | 139-279 | 0 | — | 98 | 6 |
*
Asterisks (*) represent mismatched nucleotides within the microhomology region. Junction sequences of MMEJ events containing mismatches are shown in Fig. 4.
a
The location of the junctions with respect to YEplac195 plasmid sequences.
b
The length of microhomology used during end joining.
c
The sequence of the overlapping bases. — represents events with no microhomology.
d
The number of nucleotides deleted at the 5′ PSS and 3′ PSS ends. The number of nucleotides inserted is shown in parentheses.