TABLE 2. End-Joining Events in Unirradiated and Irradiated CHO-K1 Cells.
| Junctiona | Length of microhomologyb (bp) | Overlapped basesc | Deletiond from |
||
|---|---|---|---|---|---|
| 5′ PSS end | 3′ PSS end | ||||
| Unirradiated clones | |||||
| CHO-K1-22 (ApaI) | 885-2284 | 3 | TGG | 265 | 1133 |
| CHO-K1-10 (X/B) | 962-4359 | 3 | TCT | 145 | 3205 |
| CHO-K1-14 (X/X) | 972-1144 | 2 | TG | 135 | 24 |
| CHO-K1-12 (X/X) | 692-3318 | 2 | GG | 415 | 2168 |
| CHO-K1-17 (X/X) | 1047-1167 | 2 | CG | 60 | 17 |
| CHO-K1-03 (E/X) | 820-3869 | 1 | G | 303 | 2719 |
| CHO-K1-07 (X/B) | 954-4865 | 1 | G | 153 | 3711 |
| CHO-K1-09 (X/B) | 391-4381 | 1 | G | 716 | 3227 |
| CHO-K1-18 (ApaI) | 1056-3183 | 1 | G | 94 | 2032 |
| CHO-K1-23 (ApaI) | 413-1941 | 1 | G | 737 | 790 |
| CHO-K1-05 (E/X) | 989-2947 | 1 | C | 134 | 1797 |
| CHO-K1-13 (X/X) | 513-3599 | 1 | C | 594 | 2449 |
| CHO-K1-16 (X/X) | 850-1350 | 1 | A | 257 | 200 |
| CHO-K1-01 (E/X) | 291-4356 | 0 | — | 832 | 3386 |
| CHO-K1-02 (E/X) | 452-4197 | 0 | — | 671 | 3047 |
| CHO-K1-04 (E/X) | 4-3263 | 0 | — | 1119 | 2113 |
| CHO-K1-06 (X/B) | 1094-3919 | 0 | — | 13 | 2765 |
| CHO-K1-08 (X/B) | 1141-4222 | 0 | — | 325 | 334 |
| CHO-K1-11 (X/X) | 1053-1545 | 0 | — | 54 | 395 |
| CHO-K1-15 (X/X) | 782-1484 | 0 | — | 325 | 334 |
| CHO-K1-19 (ApaI) | 594-1644 | 0 | — | 556 | 493 |
| CHO-K1-20 (ApaI) | 807-1503 | 0 | — | 343 | 352 |
| CHO-K1-21 (ApaI) | 982-1395 | 0 | — | 168 | 244 |
| Irradiated clones | |||||
| CHO-K1-IR-06 (X/B) | 402-3459 | 10 | CAAGTCCGCC | 705 | 2305 |
| CHO-K1-IR-11 (X/B) | 404-3062 | 8 | TCCGCCCC | 703 | 1098 |
| CHO-K1-IR-13 (X/B) | 675-3530 | 8 | CCCGC*CCG | 432 | 2376 |
| CHO-K1-IR-10 (X/B) | 405-3198 | 6 | CGCC*CC | 702 | 2044 |
| CHO-K1-IR-07 (X/B) | 692-4188 | 5 | GGCGG | 415 | 3034 |
| CHO-K1-IR-04 (X/B) | 242-4548 | 4 | CCGC | 865 | 3394 |
| CHO-K1-IR-05 (X/B) | 235-2044 | 4 | AAAT | 872 | 850 |
| CHO-K1-IR-08 (X/B) | 128-3511 | 3 | GAC | 979 | 2357 |
| CHO-K1-IR-02 (X/B) | 256-3530 | 3 | CCC | 851 | 2376 |
| CHO-K1-IR-01 (X/B) | 987-4306 | 0 | — | 120 | 3152 |
| CHO-K1-IR-03 (X/B) | 670-4423 | 0 | — | 437 | 3269 |
| CHO-K1-IR-09 (X/B) | 963-3732 | 0 | — | 144 | 2578 |
| CHO-K1-IR-12 (X/B) | 262-2103 | 0 | — | 845 | 949 |
*
Asterisk (*) represents a mismatched nucleotide within the microhomology region.
a
Clone name and original restriction enzymes used for linearization of pCMS-end.
b
The length of microhomology used during end joining.
c
The sequence of the overlapping bases. — represents no microhomology.
d
The number of nucleotides deleted at the 5′ PSS and 3′ PSS ends.